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. 2019 Aug 26;15(1-2):161–173. doi: 10.1080/15592294.2019.1656158

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Rat Dlgap2 gene schematic and pyrosequencing assay validation. (a) A gene schematic of Dlgap2. Exons are represented by the rectangular boxes, while the oval represents a CpG island. The sequence of interest is identified in the inset. (b) Pyrosequencing assay validation performed in triplicate for Dlgap2. Error bars represent the SEM. Error bars not shown had deviations too small for inclusion on the graph. (c) Bisulphite pyrosequencing data from control (gray) or 4mg/kg THC exposed (black) rat sperm for Dlgap2. Shown is the mean +/- SEM for each group. * = p < 0.05; ** = p < 0.01.