Figure 3.

HOXA11-AS directly binds to and targets miR-124-3p.

(a) Sequence alignment showing the binding site between miR-124-3p and wildtype HOXA11-AS (HOXA11-AS-WT), as well as the mutations made in HOXA11-AS (HOXA11-AS-MUT). (b) Cells were transfected with luciferase reporter gene driven by either HOXA11-AS-WT or HOXA11-AS-MUT. The luciferase activity was measured and compared between cells treated with miR-124-3p mimic or NC mimic. *P < 0.05. (c) and (d) qRT-PCR analysis was performed to determine the expression of HOXA11-AS and miR-124-3p. *P < 0.05, **P < 0.01.