Figure 5.

TGFβR1 is a direct target of miR-124-3p.

(a) Sequence alignment showing the binding site between miR-124-3p and wild-type TGFβR1 (TGFβR1-WT), as well as the mutations made in TGFβR1 (TGFβR1-MUT). (b) Cells were transfected with luciferase reporter gene driven by either TGFβR1-WT or TGFβR1-MUT. The luciferase activity was measured and compared between cells treated with miR-124-3p mimic or NC mimic. (c) and (d) qRT-PCR and western blot assays were performed to detect the expression of TGFβR1. *P < 0.05, **P < 0.01.