Figure 6.

Downregulation of DHRS2 causes A2780 cells to become resistant to SAHA in vivo.

A total of 3 × 10[6] A2780 cells expressing DHRS2-shRNA or an empty vector were subcutaneously injected into nude mice. SAHA (20 mg/kg/day) was administered by intraperitoneal injection and was switched on day 12, as indicated by the red arrow. A. qRT-PCR analysis of DHRS2 expression levels following stable knockdown of DHRS2. B. Tumor volumes (mm[3]) were estimated using calipers for 20 days after tumor cell injections. C. Tumors were excised at 20 days after injection and the tumor weights were measured. Data were analysed using Student’s t-test. D. Tumor photographs. E. Representative images of H&E staining and IHC for DHRS2 using tumors from mice. *p < 0.05, **p < 0.01, ***p < 0.001.