MiR-155-5p is upregulated in serum from EV71 infected patients and EV71-infected cell lines. (a) Heat map analysis of the microRNA expression between serum samples from EV71-infected patients and healthy controls (n = 3). (b) qRT-PCR was performed to measure the levels of miR-155-5p in 20 serum samples from EV71-infected patients and 10 serum samples from healthy controls. p < 0.01 vs. Control group. (c) The expression of miR-155-5p was detected by qRT-PCR in EV71-infected cell lines, including Vero, RD, HT-29, HeLa, and THP-1 cells. (d) Expression of miR-155-5p was determined in RD cells at the different times (0, 4, 6, 12, 24, 36, and 48 h) after EV71 infection. (e) Expression levels of miR-155-5p were determined in RD cells at 24 h after different dose of EV71 (0, 0.1, 0.5, 1 and 2 MOI) infection. Data are the mean ± S.D. (n = 3) of one representative experiment. *p < 0.05, **p < 0.01 vs. control group.