Figure 4.

FOXO3 is a direct target of miR-155-5p. (a) The putative binding site of miR-155-5p and FOXO3 is shown. (b) The expression levels of FOXO3 protein after transfection with miR-155-5p mimics or miR-155-5p inhibitor were measured by Western blot. (c) Luciferase activity of RD cells was detected by a dual luciferase assay. Data are the mean ± S.D. (n = 3) of one representative experiment. **p < 0.01 vs. mimics NC or inhibitor NC. (d) miR-155-5p mimics and its inhibitor were added into RD cells for 24 h, and then the cells were infected with EV71 (MOI = 1) for 24 h, and then the expression levels of FOXO3 and IRF7 protein were measured by Western blot and (e, f) blot bands were semi-quantified with ImageJ version 1.46.