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. 2019 Dec 19;19(2):179–192. doi: 10.1080/15384101.2019.1704512

Figure 5.

Figure 5.

miR-155-5p inhibition promoted EV71 replication by targeting FOXO3/IRF7 pathway. (a) Transfected efficiency of si-FOXO3 was determined by Western blot. Data are presented as means of three independent experiments ± SD. **p < 0.01 vs. si-scramble. (b) miR-155-5p inhibitor and si-FOXO3 were added into RD cells for 24 h, and then the cells were infected with EV71 for 24 h. The EV71 titers were measured by TCID50 assay. Data are the mean ± S.D. (n = 3) of one representative experiment. **p < 0.01 vs. inhibitor NC; #p < 0.05, ##p < 0.01 vs. miR-155-5p inhibitor. (c) The expression of VP1 protein was detected by Western blot analysis and blot bands were semi-quantified with ImageJ version 1.46. (d, e) The expressions of IFN-α/β at protein level were measured by ELISA. Data are the mean ± S.D. (n = 3) of one representative experiment. **p < 0.01 vs. inhibitor NC; #p < 0.05, ##p < 0.01 vs. miR-155-5p inhibitor.