Figure 3.

TWS119 treatment failed to generate a T_scm phenotype on gBT-I T cells. (a) Dot plot representing CFSE^lo proliferating gBT-I T cells in the presence of TWS119, 72 h after activation in a 6-well plate. Each datapoint represents the mean ± SD proliferation. (b) CD44 and CD62L expression of CFSE^lo T cells post-activation (top row). CFSE profile of CD44^loCD62L^hi cells demonstrates that most cells in this population remain naïve (bottom row). Plots are representative for three separate experiments. (c) TWS119-treated cells were sorted into CD44^loCD62L^hi, CD44^hiCD62L^hi populations, and untreated CD44^loCD62L^hi populations (1 x 10^{^5} cells) were transferred into a lymphodepleted animal. CD44 and CD62L expression on transferred (CD45.1) cells 4 weeks post-transfer are represented as FACS diagrams, and in (d) a dot plot.