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. Author manuscript; available in PMC: 2020 Dec 2.
Published in final edited form as: Clin Immunol. 2019 Dec 3;211:108320. doi: 10.1016/j.clim.2019.108320

Figure 3. IL-6 is secreted by human islet cultures and increased upon metabolic and immune stress.

Figure 3.

Human islets were cultured in 96-well plates. Functional GSIS assay showing insulin secretion upon low and high glucose (A), Insulin secretion upon induction of metabolic (free fatty acid and high glucose – FFA+HG) and immune stress (Proinflammatory cytokine cocktail – PIC) (B). Secretion of IL-6 by human islets with and without metabolic and immune stress (C). Multiplex immunofluorescence imaging of islets with different stressors, showing Insulin (green), Glucagon (magenta), IL-6 (red) and Hoechst (blue). Images were acquired using Zeiss LSM 780 confocal microscope.