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. 2019 Dec 28;11(1):49–56. doi: 10.1080/21505594.2019.1706913

Figure 2.

Figure 2.

Evaluation of specificity of the five sets of tet(X) [X1 to X5] primers (a). PCR assay suggests that the pair of primer [tet(X1)-F plus tet(X1)-R] is exclusively specific for tet(X1)-containing sample (b). The specificity of the primer [tet(X2)-F plus tet(X2)-R] is validated with PCR assays supplemented with the template of a single colony carrying different tet(X) gene (c). Among five kinds of different samples, only tet(X3)-bearing one is positive in PCR detection with the specific primers of tet(X3)-F plus tet(X3)-R (d). The tet(X4)-harboring sample is exclusively recognized by a single PCR with a pair of primers [tet(X4)-F plus tet(X4)-R] (e). The specificity of the primers [tet(X5)-F plus tet(X5)-R] is unique to the tet(X5)-positive sample PCR products were separated with electrophoresis of 2% agarose gel. Designations: bp, base pair; M, DNA marker (Trans 2K Plus II); the symbol of “–”, negative control.