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. 2019 Dec 28;11(1):49–56. doi: 10.1080/21505594.2019.1706913

Figure 3.

Figure 3.

Establishment of different multiplex PCR assays for the tet(X) family genes (a). Development of multiplex PCR approaches to detect different combinations of double tet(X) genes in a single colony (b). Harnessing multiplex PCR approaches for detection of three tet(X) genes in a single colony (c). Developing efficient multiplex PCR assays to detect quadruple and even quintuple variants of tet(X) from a single colony. The specific PCR products with known sizes in a single PCR act as references. Namely, it denotes 486 bp for tet(X1), 343 bp for tet(X2), 685 bp for tet(X3), 204 bp for tet(X4), and 265 bp for tet(X5) (Table 1), respectively. Two percent of agarose gel was applied to separate the mixture of PCR products. Designations: bp, base pair; M, DNA marker (Trans 2K Plus II); the symbol “–”, negative control.