Figure 1.

Enterovirus 71 Exit Cells Non-lytically In Vitro. (a) viral RNA was detected in culture supernatant after infected 1–9 h by qRT-PCR assay (mean±SD; three independent experiments). (b) infected cells incubated with SYTOX^TM Blue Nucleic Acid Stain to monitor plasma membrane intactness. c, quantification of fluorescence intensity in Figure 1(b) (mean±SD; three independent experiments; *p ≤ 0.05). Statistical analysis were performed using unpaired two-tailed student’s t-test. D, single-step growth curve of RD cells infected with EV71 at MOI of 1. Supernatants were collected from infected cells at the indicated time points, and virus titers were calculated by standard TCID₅₀ assay.