Figure 1.

Detection of Wza knockout and complementation strains.

(a) Detection of Wza knockout strains. Lane 1–4: the amplified fragment was 1269 bp, comprising the 622 bp upstream fragment and the 647 bp downstream fragment were amplified from the wza knockout strain. Lane 5: upstream and downstream regions of the wza gene were amplified from pLP-wza recombinant plasmid control. Lane 6: upstream and downstream regions of the wza gene were amplified from the WT strain, the size of the fragment was 2373 bp. Lane 7: DL2000 DNA Maker. (b) Detection of Wza complementation strains. Lane 1–4: the amplified 1259 bp fragment consists of the 1104 bp wza gene and 155 bp from the wza complementation strains. Lane 5: the amplified 1259 bp fragment consists of the 1104 bp wza gene and 155 bp from pBAD33-TC^R vector control. Lane 6: the amplified fragment of wza gene from the wza complementation strains. Lane 7: DL 2000 DNA Marker