Table 1.
List of bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
|
Acinetobacter baumannii strains |
||
| SKLX024256 (WT) | Wild-type clinical MDR isolate | Lab stock |
| WT-Δwza | WT with deletion in wza operon | This study |
| WT-C-Δwza | WT with deletion in wza operon and carrying PBAD33-TCR-wza | This study |
| ATCC 17978 | Reference strain | ATCC |
| Escherichia coli strains | ||
| DH5α | F-Φ80lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 phoA supE44λ–thi-1 gyrA96 relA1 |
Invitrogen |
| β2163 | (F−) RP4-2-Tc::Mu dapA::(erm-pir) [KmR EmR] | [56] |
| Plasmids | ||
| pLP12 | oriTRP4 oriVR6K vmi480 PBAD (tetracycline resistance) | [57] |
| pLP12-wza(Up/Down) | pLP12 containing a 622bp UP wza upstream fragment and 647bp DOWN wza downstream fragment | this study |
| PBAD33-TCR | pBAD33 vector with tetracycline resistance | [58] |
| pBAD33-TCR-wza | PBAD33-TCR carrying wza | This study |