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. 2020 Feb 1;11(1):17–30. doi: 10.14336/AD.2019.0512

Figure 3.

Figure 3.

DANCR regulates fibrogenesis by inducing FOXO3 expression. (A) Heatmap showing mRNA expression levels in RLE-6TN cells transfected with control or DANCR plasmid for 48 h. Arrow indicates FOXO3. (B) FOXO3 expression was measured by western blot analysis in cells overexpressing DANCR. (C) FOXO3 protein levels in rat lung tissues were analyzed by immunohistochemistry. (D) FOXO3 mRNA expression was detected in RLE-6TN cells (left panel) and rat lung tissues (right panel) by qRT-PCR. (E) Immunohistochemical analysis of FOXO3 levels in lung tissues treated with ASP or PBS control. (F) Western blot assay was performed to detect FOXO3 protein levels in RLE-6TN cells treated with ASP or PBS control. (G) FOXO3 expression was silenced in RLE-6TN cells by transfection with FOXO3 siRNA, **P<0.01. (H) Relative cell proliferation was measured by CCK8 assay in cells overexpressing DANCR and (or) FOXO3 knockdown cells, *P<0.05. (I) Cell migration was evaluated by Transwell assay in cells overexpressing DANCR and (or) FOXO3 knockdown cells, **P<0.01. (J) Effect of FOXO3 and DANCR on the expression of EMT-related proteins were identified by western blotting.