Table 1.
qPCR primer name | Sequence (5’-3’) |
---|---|
DANCR (Forward) | TCGGAGGTGGATTCTGTTAGG |
DANCR (Reverse) | TCGGTGTAGCAAGTCTGGTGA |
FOXO3 (Forward) | CTCCACCCCTGCTGAGATGAT |
FOXO3 (Reverse) | AGTGAGAACGTTGTCCCGCGCTGG |
GAPDH (Forward) | GCACCGTCAAGGCTGAGAAC |
GAPDH (Reverse) | ATGGTGGTGAAGACGCCAGT |
U6 (Forward) | CTCGCTTCGGCAGCACA |
U6 (Reverse) | AACGCTTCACGAATTTGCGT |
U1 (Forward) | GGGAGATACCATGATCACGAAGGT |
U1 (Reverse) Silencing RNA name |
CCACAAATTATGCAGTCGAGTTTCCC Sequence (5’-3’) |
si-AUF1 si-FOXO3 si-NC |
UCGACUAUCUGCUCCAAG TGATCAGCCTCAATCTGCA GACCTACAACTACCTATCA |