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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Science. 2018 Jun 21;361(6400):eaar2555. doi: 10.1126/science.aar2555

Fig. 3. LCD-LCD interactions involved in hub formation are highly dynamic.

Fig. 3.

(A) Snapshots of a two-color SPT movie simultaneously imaging EYFP-labeled (green) EWS LCD-LacI (top, forming a LacO-associated LCD hub) or EWS LCD (bottom, forming self-aggregated LCD hubs not affiliated with the LacO array) and Halo-tagged EWS LCD (2 nM PA-JF646 labeled, red) in U2OS cells containing LacO array 1. A white dashed contour outlines the cell nucleus. We imaged the hubs in the EYFP channel (green) and tracked individual Halo-EWS LCD molecules with an acquisition time of 500 ms in the PA-JF646 channel (red). (B) Residence times of LCD (red) bound at the LacO-array-associated LCD hub or at self-aggregated LCD hubs not affiliated with the array (green). *P < 0.05, two-sample t test. Error bars represent SE.

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