| PCR1 mix | |
|---|---|
| Component | Volume (uL) |
| Nuclease-free water | 4 |
| 5X Phusion HF Buffer | 4 |
| 2.5 mM dNTPs | 1.6 |
| 100 μM PCR1fwd | 0.1 |
| 100 μM PCR1rev | 0.1 |
| Template gDNA | 10 |
| Phusion DNA Polymerase | 0.2 |
| TOTAL VOLUME | 20 |
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