| PCR2 mix | |
|---|---|
| Component | Volume (μL) |
| Nuclease-free water | (variable) |
| 5X Phusion HF or GC Buffer | 40 |
| 2.5 mM dNTPs | 16 |
| 100 μM PCR2fwd | 1 |
| 100 μM PCR2rev | 1 |
| Template DNA | (variable, 40 ng) |
| Phusion DNA Polymerase | 2 |
| TOTAL VOLUME | 200 |
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