(A) Changes in volume-corrected fluorescence intensity (Fcorr) upon titration with paromomycin, 4′-O-ethylparomomycin, and propylamycin are shown. (B) Representative binding curves of fraction bound (Fr) as a function of paromomycin, 4′-O-ethylparomomycin, and propylamycin concentration fitted to Equation 3 are overlaid (R2 = 0.99, 0.97, and 0.95, respectively).