Table. 1.

Nucleic acids for the synthesis of RGD-null ITS that reports their DNA resistance to DNases. (Sequences are from 5‵ end to 3‵ end)

1	DNA upper strand for RGD-null ITS	GGG CGG CGA CCT CAG CAT/3BHQ_2/
2	DNA lower strand for RGD-null ITS	/5BiosG/T/iCy3/ATG CTG AGG TCG CCG CCC/
3	RNA upper strand for RGD-null ITS	mG*mG*mG* mC*mG*mG* mC*mG*mA* mC*mC*mU* mC*mA*mG* mC*mA*mU*/3BHQ_2/
4	RNA lower strand for RGD-null ITS	/5BiosG/mU*/iCy3/mA*mU*mG* mC*mU*mG* mA*mG*mG* mU*mC*mG* mC*mC*mG* mC*mC*mC*/
5	PNA lower strand for RGD-null ITS	Biotin-OO-Lys(Cy3)-O-ATGCTGAGGTCGCCGCCC

Notes:

^1.Strands 1 and 2 were hybridized to dsDNA-based RGD-null ITS.

^2.Strands 3 and 4 were hybridized to dsRNA-based RGD-null ITS.

^3.Strands 1 and 5 were hybridized to PNA/DNA-based RGD-null ITS.

^4.Strands 3 and 5 were hybridized to PNA/RNA-based RGD-null ITS.

^5./3BHQ_2/ represents a Black Hole Quencher 2 at the 3’ end.

^6./5BiosG/ represent a biotin tag at the 5’ end of a nucleic acid.

^7.m_* in strands 5 and 6 represents a phosphorothioated 2’O-methyl base.

^8.“O” in the PNA sequence represents an ethylene glycol that increases the PNA solubility in water.