Table. 2.

Nucleic acids for the synthesis of ITS reporting cellular forces. (Sequences are from 5‵ end to 3‵ end)

1	DNA upper strand for ITS	/5ThioMC6-D/GGG CGG CGA CCT CAG CAT/3BHQ_2/
2	DNA upper strand for ITS (Linked with RGD)	/RGD/GGG CGG CGA CCT CAG CAT/3BHQ_2/
3	DNA lower strand for ITS	/5Cy3/ATG CTG AGG TCG CCG CCC/3Bio/
4	RNA upper strand for ITS	/5ThioMC6-D/ mG*mG*mG* mC*mG*mG* mC*mG*mA* mC*mC*mU* mC*mA*mG* mC*mA*mU*/3BHQ_2/
5	RNA upper strand for ITS (Linked with RGD)	/RGD/ mG*mG*mG* mC*mG*mG* mC*mG*mA* mC*mC*mU* mC*mA*mG* mC*mA*mU*/3BHQ_2/
6	RNA lower strand	/5Cy5/mA*mU*mG* mC*mU*mG* mA*mG*mG* mU*mC*mG* mC*mC*mG* mC*mC*mC*/3Bio/
7	PNA lower strand	Cy3-O-ATGCTGAGGTCGCCGCCC-KKKK(Biotin)

Notes:

^1./5ThioMC6-D/ represents a thiol modification which is used to conjugate a RGD peptide to a DNA or a RNA. Protocol of conjugating RGD to DNA strand 1 or RNA strand 6 can be found in (Zhao et al., 2019).

^2./3Bio/ represent a biotin tag at the 3’ end of a nucleic acid.

^3.“K” in the PNA sequence represents a lysine that increases the PNA solubility in water.

^4.Strands 2 and 3 were hybridized to dsDNA-based RGD ITS.

^5.Strands 5 and 6 were hybridized to dsRNA-based RGD ITS.

^6.Strands 2 and 7 were hybridized to PNA/DNA-based RGD ITS.

^7.Strands 5 and 7 were hybridized to PNA/RNA-based RGD ITS.