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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Virology. 2019 Nov 25;540:150–159. doi: 10.1016/j.virol.2019.11.012

Fig. 3. Downstream STING signaling is blocked in LEC4 cells.

Fig. 3.

(A) BEC3 and LEC4 were transfected with either 1 μg/mL, Poly I:C, 10 μg/mL ISD 100mer or 5 μg/mL, 2′3′ cGAMP for 3 h and whole cell lysates were immunoblotted with the indicated phospho-specific or total protein antibodies. (B) BEC3 and LEC4 were transfected with 5 μg/mL 2′3′ cGAMP and cells were harvested at the indicated time points and whole cell lysates were immunoblotted in native (nonreducing) sample buffer for the indicated antibodies.