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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Virology. 2019 Nov 25;540:150–159. doi: 10.1016/j.virol.2019.11.012

Fig. 6. STING does not reduce susceptibility during de novo infection or restrict spread of KSHV during lytic replication.

Fig. 6.

(A) LEC4 and LEC8 cells were infected with KSHV-BAC16 and infection rates were measured by immunofluorescence microscopy and quantifying the percent of LANA + cells in the culture at 48 hpi. (B) BEC1-3 were transduced with a lentivirus expressing Cas9 and a guide RNA targeting STING (ΔSTING) or a nontargeting (scramble) control and whole cell lysates were immunoblotted with the indicated antibodies. (C) Scramble and ΔSTING BEC3 from (B) were infected with KSHV-BAC16 and IFN-β mRNA was measured by RT-qPCR at 48 hpi as described earlier. (D) Scramble and ΔSTING BEC1-3 were infected with KSHV-BAC16 at different dilutions of virus and infection rates were measured by immunofluorescence microscopy and quantifying the percent of LANA + cells in the culture at 48 hpi. (E) Scramble and ΔSTING BEC3 were infected with KSHV-BAC16 at 2 different dilutions (1:140 and 1:120) for 4 h. Immediately following infection, cells were treated with PMA for 5 days. Cells were sorted by GFP by flow cytometry. Data are shown as mean ± SEM from at least 2 biological replicates.