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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Cell Rep. 2019 Dec 10;29(11):3751–3765.e5. doi: 10.1016/j.celrep.2019.11.026

Figure 2. Identification of Noncanonical Isoforms in the Human Proteome.

Figure 2.

(A) Comparison on the number of sequences in standard databases (RefSeq TrEMBL, SwissProt canonical + isoform, and SwissProt canonical) versus the custom tissue-specific databases. The custom databases have fewer sequences than SwissProt

(B) The proportion of distinct peptides uniquely mappable to noncanonical isoforms per tissue, with the heart and testis particularly enriched in noncanonical isoforms. Color of data points corresponds to each of 5 reanalyzed human proteome datasets.

(C) Proportion of AS types in RNA-seq data (left) compared to identified noncanonical peptides (right), showing higher translatable rate for MXE.

(D) The number of uniquely identified noncanonical junction peptides at 1% FDR across tissues in 5 reanalyzed human proteome datasets (ProteomeXchange: PXD000561, PXD009737, PXD009021, PXD006675, and PXD010154), including noncanonical sequences from known isoforms and undocumented sequences. Color: AS type (A3SS, A5SS, MXE, RI, and SE).