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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Cell Physiol. 2019 Oct 6;235(4):3731–3740. doi: 10.1002/jcp.29267

Figure 1. SOX2 elevation inhibits the proliferation of androgen-dependent LNCaP cells.

Figure 1.

A. Western blot analysis of SOX2 overexpression in i-SOX2-LNCaP whole cell extracts following growth with or without Dox. HDAC1 was used as a protein loading control. B. Cell proliferation of i-SOX2-LNCaP cells was determined by MTT assay following growth at 3, 5, and 7 days in the absence and presence of Dox at the indicated concentration. Relative proliferation was normalized to the control at each time-point. C. MTT analysis of parental (unengineered) LNCaP cells after 4 days growth in the presence of Dox (100 ng/ml). D. Clonal growth of i-SOX2-LNCaP cells in the presence and absence of Dox (100 ng/ml) after 8 days in culture. Twenty random fields were scored for each treatment. Error bars represent standard deviation; statistical significance was determined by a 2-tailed student’s t-test (N.S. not significant,*p<.05,**p<.01,***p<.005).