Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Cell Physiol. 2019 Oct 6;235(4):3731–3740. doi: 10.1002/jcp.29267

Figure 2. Elevating SOX2 is sufficient to induce neuroendocrine plasticity in i-SOX2-LNCaP cells.

Figure 2.

A. RT-qPCR analysis of AR target genes (PSA, NKX3.1, TMPRSS2) and neuroendocrine markers (BRN2, NTS, GRP, CALCA) in i-SOX2-LNCaP cells after 48 hrs growth in the presence or absence of Dox (100 ng/ml). B. Western blot analysis of SYP and AR expression in i-SOX2-LNCaP whole cell extracts grown in the presence and absence of Dox (100 ng/ml) for 48 hrs. HDAC1 and β-tubulin were used as protein loading controls. Error bars represent standard deviation; statistical significance was determined by a 2-tailed student’s t-test (*p<.05,**p<.01,***p<.005).