Fig 3. ST2-expressing basophils are required for ADTI responses.

(A) Representative gating for basophils (MCPT8-YFP+) and (B) quantification of total cells and basophils in PBS and DNP-IgE/DNP-HSA treated ears 2 hours after challenge. (C) WT x iDTR (control) and Basoph8 x iDTR mice received 3 consecutive doses of DT (1 μg) for basophil depletion. Mice then underwent the PCA model as described in the methods. Ear thickness was measured at 0, 1 and 24 hours. (D) C57BL/6 WT and St2^–/–mice received WT or St2^–/–BMBs, and the PCA model was performed 1 hour later. Ear thickness (D) was measured at 0, 1, and 24 hours after challenge, and ear neutrophil population (CD45⁺Gr-1^highSiglec-F^low) was analyzed by flow cytometry (E). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001 (one-way ANOVA). Data from (A, B) are from at least 2 independent experiments. Data from (C, D) are from at least 4 independent experiments, and the mean ± SEM from n = 11 (B), n = 9–17 (C), n = 12–17 (D) and n = 7–15 mice per group (E) are displayed.