Table of Materials
| Name of Material/ Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| Reagents and Material | |||
| 60mm × 15mm Petri Dish | Fisher | FB0875713A | |
| Agar | Fisher | BP1423–500 | |
| Sodium Chloride | Fisher | S671–3 | |
| Peptone | Fisher | BP1420–500 | |
| Cholesterol | Sigma-Aldrich | C8667 | |
| LB broth, Miller | Fisher | 1426–2 | |
| Escherichia coli strain NA22 | Caenorhabditis Genetics Center (CGC) | NA22 | Either this or OP50 E. coli can be used for C. elegans maintenance and assay. Both may be purchased at the CGC |
| N2 | CGC | N2 | |
| fog-2(q71) | CGC | CB4108 | |
| Platinum wire 0.25mm dia | Alfa Aesar | 10288 | |
| 5 3/4” Disposable Pasteur pipet | Fisher | 13-678-20A | |
| Watch glass | Fisher | 02–612A | |
| 5mm Dia. Glass rod | Fisher | 50-121-5269 | |
| MitoTracker CMXRos | Fisher | M7512 | Shield from light, store at −20℃ |
| Monopostassium phosphate | Fisher | P285-500 | |
| Disodium phosphate | Fisher | S374-1 | |
| Magnesium sulfate | Fisher | M63–500 | |
| Dimethyl sulfoxide | Fisher | BP231–1 | DMSO |
| Aluminum foil | Fisher | 01-213-102 | |
| Ethyl 3-aminobenzoate methanesulfonate | Sigma | E10521-10G | Tricaine is the common name |
| Tetramisole hydrochloride | Sigma | L9756-5G | |
| Agarose | Fisher | BP1356–100 | |
| Coverslips | Fisher | 12-548-A | 18 × 18-1 |
| Frosted microscope slides | Fisher | 12-552-3 | |
| Equipment | |||
| 16℃ and 20℃ incubators | Fisher | 97–990E | Same model, set at different temperatures. |
| Upright Microscope with epi-fluorescence illuminator, camera, and 10x and 60x objectives | Refer to the protocol section for specific model information | ||
| Software with image acquisition and tracking capabilities | Protocol given based on the NIS-Elements software | ||
| Stereo-microscope | Nikon | SMZ800N | Any stereo-microscope that can be used to visualize C. elegans may be used with this protocol |