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. 2019 Dec 23;8:e52503. doi: 10.7554/eLife.52503

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© 2019, Murase et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) Representative triple labeled fluorescent micrographs of Wisteria floribunda agglutinin (WFA)-FITC staining (cyan), immunostaining for aggrecan (Agg; yellow) and parvalbumin (PV; magenta) in deprived (dep) and non-deprived (non) V1b in cMD (left) and cMD+LRx subjects (LRx, right). Roman numerals indicate cortical layer. WM = white matter. Inset: High magnification images of triple labeled PV⁺ interneurons (100X). (B) Fluorescence intensity profiles (mean ± SEM) along vertical depth of V1b. cMD dep (dark blue), cMD non (light blue), LRx dep (dark red), LRx non (red). (C) A parallel and significant decrease in WFA and Agg mean fluorescence intensity 250–400 μm from surface in dep and non V1b following LRx. One-way ANOVAs, WFA F_{(3, 26)}=32, p=0.0001; Agg F_{(3, 26)}=10, p=0.0001; PV F_{(3, 26)}=0.4, p=0.75; n = 7, 7, 8, eight subjects for cMD dep, cMD non, LRx dep, LRx non, respectively; *p<0.05, Tukey-Kramer post hoc test. (D) LRx induces a significant decrease in WFA and Agg fluorescence intensity at PV⁺ and PV^-locations in dep and non V1b. One-way ANOVAs, WFA PV⁺, F_{(3, 309)}=40.3, p<0.0001; WFA PV^-, F_{(3, 309)}=30.1, p<0.0001; Agg PV⁺, F_{(3, 309)}=29.4, p<0.0001; Agg PV^-, F_{(3, 309)}=18.7, p<0.0001; n (subjects, ROIs)=(5, 77 , 5, 73 , 5, 81 , 5, 82), for cMD dep, cMD non, LRx dep, LRx non, respectively; *p<0.05, Tukey-Kramer post hoc test.

Figure 2—source data 1. Source data for Figure 2.

elife-52503-fig2-data1.xlsx^{(203.4KB, xlsx)}

Figure 2—figure supplement 1. — (A) Representative triple labeled fluorescent micrographs of Wisteria floribunda agglutinin (WFA)-FITC staining (cyan), immunostaining for aggrecan (Agg; yellow) and parvalbumin (PV; magenta) in deprived (dep) and non-deprived (non) V1b in cMD (left) and cMD+LRx subjects (LRx, right). Roman numerals indicate cortical layer. WM = white matter. Inset: High magnification images of triple labeled PV⁺ interneurons (100X). (B) Fluorescence intensity profiles (mean ± SEM) along vertical depth of V1b. cMD dep (dark blue), cMD non (light blue), LRx dep (dark red), LRx non (red). (C) A parallel and significant decrease in WFA and Agg mean fluorescence intensity 250–400 μm from surface in dep and non V1b following LRx. One-way ANOVAs, WFA F_{(3, 26)}=32, p=0.0001; Agg F_{(3, 26)}=10, p=0.0001; PV F_{(3, 26)}=0.4, p=0.75; n = 7, 7, 8, eight subjects for cMD dep, cMD non, LRx dep, LRx non, respectively; *p<0.05, Tukey-Kramer post hoc test. (D) LRx induces a significant decrease in WFA and Agg fluorescence intensity at PV⁺ and PV^-locations in dep and non V1b. One-way ANOVAs, WFA PV⁺, F_{(3, 309)}=40.3, p<0.0001; WFA PV^-, F_{(3, 309)}=30.1, p<0.0001; Agg PV⁺, F_{(3, 309)}=29.4, p<0.0001; Agg PV^-, F_{(3, 309)}=18.7, p<0.0001; n (subjects, ROIs)=(5, 77 , 5, 73 , 5, 81 , 5, 82), for cMD dep, cMD non, LRx dep, LRx non, respectively; *p<0.05, Tukey-Kramer post hoc test.

Figure 2—source data 1. Source data for Figure 2.

elife-52503-fig2-data1.xlsx^{(203.4KB, xlsx)}