Table 8.

Prevalence (%) of neurons with significant expression of genes that covary with fast-firing capacity in MVN cell types

	Pre-oculomotor	Pre-cerebellar	Unknown	Commissural	Cerebellar target	Local interneuron
Cacna1a	100	95	100	100	85	86
Cplx1	100	100	100	100	100	100
Kcna1	100	100	50	87	85	47
Kcnc1	100	100	100	100	100	99
Kcng4	100	90	0	90	15	31
Kcnh2	100	95	100	100	92	91
Nefh	100	100	100	89	54	33
Nefl	100	100	100	100	100	100
Nefm	100	100	100	100	54	72
Scn1a	100	100	83	97	100	95
Scn1b	100	100	100	100	100	100
Scn4b	100	100	33	80	0	5
Scn8a	100	100	100	100	92	92
Syt2	100	95	33	70	15	1
Vamp1	100	100	100	100	100	100

Of 134 candidate genes the transcript levels of which potentially covary with fast-firing capacity of MVN neurons, 15 genes were confirmed for highly correlative expression (average r > 0.6) with the fast-firing ion channels across individual MVN samples. The percentage of cells showing significant expression of the genes are shown for each cell type. Cacna1a and Kcng4 are included in the list because, although the microarray failed to detect them, the high correlation of its expression to the fast-firing genes were confirmed at the single-cell level (Figure 4A,B). For Cplx1, Nefh, Nefl, Nefm, and Vamp1, 102 cells were examined (pre-oculomotor, n = 20; pre-cerebellar, n = 19; unknown, n = 6; commissural, n = 26; cerebellar target, n = 13; local interneuron, n = 18). For all the other genes, 167 cells were examined (pre-oculomotor, n = 21; pre-cerebellar, n = 20; unknown, n = 6; commissural, n = 30; cerebellar target, n = 13; local interneuron, n = 77).