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. 2020 Jan 15;40(3):542–556. doi: 10.1523/JNEUROSCI.0915-18.2019

Figure 8.

Figure 8.

Rab11 regulates R-neuron axonal branching downstream of CCB. Axonal branches of R-neurons innervating the adult EB. Flies expressing either the constitutively active form of Rab11 (A, C), Rab11Q70L (UAS-Rab11CA::YFP/+; c507-Gal4/+), or the dominant-negative (B, D), Rab11S25N (UAS-Rab11DN::YFP/+; c507-Gal4/+), alone (A, B) or in combination with the ccbRNAi (C, D) under the control of the c507-Gal4 driver. E, Quantification of the YFP signal in the EB. YFP pixel intensity was significantly lower in R-neurons expressing the dominant-negative form of Rab11 compared with the constitutively active one (Kruskal–Wallis, p = 0.0185). This effect was enhanced when coexpressing the ccbRNAi and Rab11DN (Kruskal–Wallis, p = 0.0079); however, downregulation of ccb did not affect Rab11CA-induced branching in the EB (Kruskal–Wallis, p = 0.8999). Scale bar, 30 μm. Error bars indicate SEM. *p < 0.05, **p < 0.01; n = 8–12 per group. n.s. = not significant.