Table 1.
Antibodies
| Antibody | Dilution | Catalog no. (manufacturer) |
|---|---|---|
| Mouse anti-cFos (monoclonal) | 1:1000 | sc-166940 (Santacruz Biotech) |
| Guinea pig anti-NeuN (polyclonal) | 1:2000 | ABN90 (Millipore Sigma) |
| Sheep anti-TH (polyclonal) | 1:1000 | AB1542 (Millipore Sigma) |
| Rabbit anti-TPH2 (polyclonal) | 1:500 | PA1–778 (ThermoFisher Scientific) |
| Guinea pig anti-VGLUT3 (polyclonal) | 1:100 | AB5421-I (Millipore Sigma) |
| Sheep anti-bNOS (polyclonal) | 1:500 | AB1529 (Millipore Sigma) |
| Donkey anti-sheep AlexaFluor 647 (secondary) | 1:500 | A21448 (Invitrogen) |
| Donkey anti-sheep AlexaFluor 488 (secondary) | 1:500 | A11015 (Invitrogen) |
| Donkey anti-guinea pig AlexaFluor 488 (secondary) | 1:500 | 706–545-148 (Jackson ImmunoResearch) |
| Donkey anti-rabbit AlexaFluor 555 (secondary) | 1:500 | A31572 (Invitrogen), SAB4600177 (Sigma-Aldrich) |
| Horse anti-rabbit biotinylated (secondary) | 1:300 | BA-1100 (Vector Laboratories) |
| Donkey anti-mouse AlexaFluor 647 (secondary) | 1:500 | A31571 (Invitrogen) |
Primary and secondary antibodies used in this study listed with details of host and target species, type (polyclonal, monoclonal, or secondary), working dilution, and product supplier.