Figure 4. Characteristics of analytes selected by tool.

Sensitivity analysis shows that, even with cross-validation implemented in LASSO, 5/17 analytes are not robust to different parameter settings within the tool. These 5, indicated in grayscale, are unlikely to be validated in an independent cohort. Each individual plot’s x and y axes represent settings used to run the analytical tool; the number of analytes per assay setting is on the x axis, and minimum correlation per cluster is on the y axis of each miniplot. Darkest coloring indicates that the analyte was selected by the tool using that combination of x and y settings. Lighter coloring indicates that another analyte in that same cluster was selected by the tool. Light gray indicates that this analyte was not selected using that combination of x and y settings. Each miniplot is labeled by analyte and the assay from which it was originally measured. “Affy” indicates the transcriptional response to T1D serum assay as this is conducted on the Affymetrix platform.