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. 2019 Dec 5;4(23):e127868. doi: 10.1172/jci.insight.127868

Figure 3. Nfat5 overexpression prevented osteoclastogenesis of RANKL/M-CSF–treated RAW264.7 cells.

Figure 3

(A) Nfat5 mRNA expression in RAW264.7 cells without (Nfat5-WT) or with Nfat5 overexpression (Nfat5-over, n = 6). Representative NFAT5 immunoblot. (B) Expression of osteoclast-specific genes in Nfat5-WT or Nfat5-over cells (n = 6). (C) Representative TRAP staining (red) of Nfat5-WT or Nfat5-over cells and TRAP assay of the supernatants (n = 9). (D) Representative images of CaP resorption assay. Resorbed CaP areas appear as black gaps. Quantification of CaP resorption assay using ImageJ (n = 9). AU, arbitrary units. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Welch-corrected ANOVA with Games-Howell post hoc tests. Scale bar: 100 µm.