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. 2019 Dec 5;4(23):e127868. doi: 10.1172/jci.insight.127868

Figure 6. NFAT5 regulates OPG expression under HS¢ in murine osteoblasts.

Figure 6

NFAT5 influences OPG expression under high-salt conditions (HS¢) in murine osteoblasts. (A) NFAT5 mRNA and protein expression in ns-siRNA– or Nfat5 siRNA–treated osteoblasts under NS¢ or HS¢ (n = 6). Representative NFAT5 immunoblot of ns-siRNA– or Nfat5 siRNA–treated samples under NS¢ or HS¢. (B) OPG mRNA and protein expression in ns-siRNA– or Nfat5 siRNA–treated osteoblasts under NS¢ or HS¢ (n = 6 per group). (C) ChIP analysis of interaction between NFAT5 and Opg promotor in osteoblasts under NS¢ or HS¢ (n = 4). AU, arbitrary units. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Welch-corrected ANOVA with Games-Howell post hoc tests, except C (unpaired, 2-tailed Student’s t test).