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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Commun Chem. 2019 Sep 19;2:111. doi: 10.1038/s42004-019-0211-7

Fig. 3.

Fig. 3

Installation of the ligation auxiliary hdmb onto NEMO298–308. a Scheme for direct addition of the auxiliary to the lysine side-chain, conditions: (i) Pd (PPh3)4, PhSiH3, DCM (ii) 3, DMF (iii) NaBH4, DMF (iv) TFA/TES/EDT/H2O (v) TFA/TMSBr/thioanisole/EDT. b Analytical HPLC of crude peptides and MALDI-TOF MS of pure peptides at stages of auxiliary addition. H-ADIYK(Alloc)ADFQAE-NH2: m/z = 1353.8 [M + H]+, Calc. = 1353.6. H-ADIYK(hdmb-Mob)ADFQAE-NH2: m/z = 1587.8 Calc. = 1587.7 (a metastable ion is also observed m/z = 1301.0). H-ADIYK(hdmb)ADFQAE-NH2: m/z = 1467.9 [M + H]+, Calc. = 1467.6