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Chemical ubiquitylation of NEMOCoZi with hdmb auxiliary-assisted ligation. a Time-course for auxiliary ubiquitylation of NEMOCoZi monitored by gel electrophoresis (lane 1–6). Lane 7 is the ligation product after hdmb removal and HPLC purification. b MALDI-TOF MS of NEMOCoZi-Ub m/z = 18688 [M + H]+, calc. Av. m/z = 18690
