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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Commun Chem. 2019 Sep 19;2:111. doi: 10.1038/s42004-019-0211-7

Fig. 6.

Fig. 6

Structural characterisation of NEMOCoZi and NEMOCoZi-Ub by circular dichroism spectroscopy. a Far-UV CD spectra of NEMOCoZi, NEMOCoZi-Ub and ubiquitin measured at 20 °C. The CD spectra of NEMOCoZi-Ub is very similar to the the sum of the spectra of the individual components, NEMOCoZi and ubiquitin. b Far-UV CD spectra of NEMOCoZi (16 μM) at different temperatures. c Thermal melting curves of NEMOCoZi measured at 222 nm. The melting temperature (grey arrows) increases at higher concentrations of NEMOCoZi, from 20.2 °C, to 24.9 °C, and to 28.5 °C