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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Commun Chem. 2019 Sep 19;2:111. doi: 10.1038/s42004-019-0211-7

Fig. 7.

Fig. 7

Biophysical characterisation and substrate properties of NEMOCoZi-Ub a Biolayer interferometry measurement of binding of linear di-Ub to NEMOCoZi and NEMOCoZi-Ub. Data points from four independent experiments are shown. Errors on the dissociation constants represent the standard error from non-linear least square fitting to the combined datasets. b model of the NEMOCoZi-Ub coiled-coil, time-course of linear ubiquitylation of NEMOCoZi-Ub by the RBR domain of HOIP (HOIP) and E1 and E2 ligases with N-terminal His-tagged ubiquitin (His-Ub) showing the emergence of di-ubiquitylated NEMOCoZi (NEMO-Ub2)