Table 1.
EBV carriage renders the cells more sensitive to inhibition of MTH1
| % Apoptotic cellsa | Fold changef | |||||
|---|---|---|---|---|---|---|
| Cell lines | EBVb | Control | TH588c | S-crizotinibd | TH588 | S-crizotinib |
| BJAB | − | 7.7 ± 1.7 | 12.2 ± 0.1 | 15.6 ± 1.4 | 1.6 | 2.0 |
| BJAB-B958 | + | 8.1 ± 4.1 | 32.7 ± 3.4 | 39.9 ± 1.4 | 4.0 | 4.9 |
| BL41 | − | 5.4 ± 1.0 | 19.3 ± 4.4 | 22.9 ± 4.7 | 3.6 | 4.3 |
| BL41-E95B | + | 14.8 ± 5.5 | 88.0 ± 4.0 | 94.5 ± 0.9 | 6.0 | 6.4 |
| Akata | − | 4.8 ± 1.1 | 30.3 ± 2.3 | 11.7 ± 3.8 | 6.3 | 2.4 |
| Akata-BX1 | + | 3.2 ± 1.5 | 51.9 ± 2.9 | 7.0 ± 1.2 | 16.4 | 2.2 |
| AGS | − | 1.6 ± 0.2 | 14.3 ± 2.7 | 3.8 ± 0.3 | 8.9 | 2.4 |
| AGS-BX1 | + | 1.7 ± 0.8 | 26.5 ± 0.9 | 11.2 ± 0.5 | 15.3 | 6.5 |
| TWO3 | − | 9.8 ± 1.7 | 27.6 ± 4.6 | 19.2 ± 2.4 | 2.8 | 2.0 |
| TWO3-EBV | + | 12.5 ± 4.5 | 25.6 ± 1.7 | 54.7 ± 7.0 | 2.0 | 4.4 |
| eEBNA1 | ||||||
| BJAB | − | 7.7 ± 1.7 | 12.2 ± 0.1 | 15.6 ± 1.4 | 1.6 | 2.0 |
| BJAB-EBNA1 | + | 4.8 ± 1.0 | 31.4 ± 2.8 | 21.7 ± 2.5 | 6.5 | 4.5 |
| BJAB-tTAE1 Dox+ | − | 9.0 ± 2.5 | 19.6 ± 0.2 | 23.8 ± 1.0 | 2.2 | 2.7 |
| BJAB-tTAE1 Dox- | + | 9.7 ± 2.3 | 40.4 ± 1.2 | 48.1 ± 0.8 | 4.2 | 5.0 |
Values corresponding to ≥ 50% increased apoptosis in EBV/EBNA1 positive cells are underlined
aApoptosis was measured by staining with PI/Annexin-V and flow cytometry analysis. The mean ± SD positive cells in two independent experiments is shown
bEBV carriage was in all cases confirmed by detection of EBNA1
cThe cells were treated with 10 μM TH588 for 48 h
dThe cells were treated with 5 μM S-Crizotinib for 48 h
eEBNA1 expression was confirmed by western blot analysis
f Fold change was calculated as the ratio between the mean % apoptotic cells in control and treated cells