Fig. 2.

TR4 can influence renal cell carcinoma sensitivity to sunitinib via altering AXL expression. Under TR4 overexpressed and knocked down conditions, the expression of AXL changes along with TR4 at mRNA level in OSRC-2 (a) and SW839 (b) cells. Similar results are shown in protein level by Western blot (left) with quantifications (right) in OSRC-2 (c) and SW839 (d) cells. e TR4 binding profile map predicted by JASPAR. f Speculated potential promoter structure of AXL binding to the TR4 response element (TR4RE). g ChIP assay shows the binding capacity of TR4REs to the speculated promoter structure of AXL under the condition of normoxia, hypoxia and TR4 overexpression in OSRC-2 cells. In a and b, data is presented as mean ± s.e.m., *p < 0.05, **p < 0.01