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. 2020 Jan 9;10:2945. doi: 10.3389/fimmu.2019.02945

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Copyright © 2020 Gan, Zhang, Gu, Ju, Wu, Wang, Peng, Qiu, Zhou, Cheng and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acidic microenvironment decreases CD4⁺CD25⁺Foxp3⁺ iTreg induction by proapoptosis. (A) Induction efficiency of CD4⁺CD45RA⁺ naïve T cells cultured with IL-2 and TGF-β for 9 days at the indicated pH values. (B) Statistical analysis of induction efficiency. Data are presented as the means ± SD from three independent experiments. NS, not significant. *p < 0.05; ***p < 0.001.