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. 2020 Jan 9;10:2945. doi: 10.3389/fimmu.2019.02945

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Copyright © 2020 Gan, Zhang, Gu, Ju, Wu, Wang, Peng, Qiu, Zhou, Cheng and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Omeprazole reverses the decreased iTreg differentiation caused by acidic pH. (A) Induction efficiency of CD4⁺CD45RA⁺ naïve T cells cultured with varying concentrations of omeprazole for 3 days at the indicated pH values. (B) Statistical analysis of induction efficiency of different groups. (C) Cells of different groups were harvested and subjected to SDS-PAGE and Western blot with the indicated antibodies. Data are presented as the means ± SD from three independent experiments. NS, not significant. **p < 0.01; ***p < 0.001.