Figure 7.

Buffering of acidic microenvironment increases Treg infiltration and alleviates liver injury in HIRI mouse model. (A,B) Serum ALT and aspartate transaminase (AST) levels in the I/R, NaHCO₃ treatment, and sham mouse groups by time points. (C) Representative light microscopy images of mouse liver injury (HE staining, 200×) in the I/R, NaHCO₃ treatment, and sham groups by time point. (D) Representative light microscopy images of mice liver immunohistochemical staining in the I/R, NaHCO₃ treatment, and sham groups (brown: positive cells, anti-Foxp3, 400×) by time point. (E) pH values of injured liver lobe after reperfusion in I/R, NaHCO₃ treatment, and sham mouse groups by time point. (F) Statistical analysis of Foxp3⁺ cell relative ratio of I/R, NaHCO₃ treatment, and sham mouse groups in immunohistochemical staining. (G) Suzuki scores evaluating liver biopsies from different groups, as performed by an experienced pathologist. Data are presented as the means ± SD from three independent experiments. NS, not significant. *p < 0.05; **p < 0.01; ***p < 0.001.