Figure 8.

Reversal of acidic microenvironment attenuates HIRI in mice via Treg function. (A,B) Serum ALT and aspartate transaminase (AST) levels of mice in sham, I/R, NaHCO₃ treatment, and NaHCO₃ + PC61 treatment groups after reperfusion for 6 h. (C) Representative images of mouse liver injury (HE staining, 200×; immunohistochemical staining, 400×) in sham, I/R, NaHCO₃ treatment, and NaHCO₃ + PC61 treatment groups by light microscopy after reperfusion for 6 h. (D) Suzuki scores evaluating liver biopsies from different groups, as performed by an experienced pathologist. (E) Statistical analysis of Foxp3⁺ cells relative ratio of sham, I/R, NaHCO₃ treatment, and NaHCO₃ + PC61 treatment groups in immunohistochemical staining. Data are presented as the means ± SD from three independent experiments. NS, not significant. **p < 0.01; ***p < 0.001.