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. 2020 Jan 15;10:357. doi: 10.1038/s41598-019-57047-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BMI1 re-expression in cancer cells with SOX9 knockdown restores tumor growth in vivo. (A) Volume at the indicated time points of the tumors generated after subcutaneous injection of 1 × 10⁵ MKN45 pLKO GFP, sh1 GFP or sh1 BMI1 cells in nude mice (n = 12 injections/ condition). (B) Picture of representative tumors generated in (A). (C) Average volume of the subcutaneous tumors generated by the injection of 0.5 × 10⁶ Panc-1 cells with SOX9 silencing (sh1 GFP) and Panc-1 cells with SOX9 silencing and BMI1 restoration (sh1 BMI1) in nude mice (n = 8 injections/condition). (D) Representative images of the hematoxilin-eosin (HE) staining and immunohistochemical staining of SOX9, BMI1, p21^CIP and Ki67 in tumors from (A). Asterisks (*, ** and ***) indicate statistical significance (p < 0.05, p < 0.01, and p < 0.001, respectively).