Table 1.
Antibodies and dilution used.
| Antigen | Species of origin | Dilution | Supplier |
|---|---|---|---|
| Syndecan 1 (CD138) | Mouse | 1:100 | Dakocytomation |
| Syndecan 2 | Rabbit | 1:250 | Santa cruz biotechnology |
| Syndecan 3 | Goat | 1:50 | Santa cruz biotechnology |
| Glypican 1 | Rabbit | 1:100 | Thermoscientific |
| Perlecan | Rabbit | 1:100 | Santa cruz biotechnology |
| Agrin | Goat | 1:100 | Santa cruz biotechnology |
| TGFβ RIII | Mouse | 1:100 | Santa cruz biotechnology |
| HS (10E4 epitope) | Mouse | 1:100 | Amsbio |
| CS (Clone CS-56) | Mouse | 1:100 | Sigma-aldrich corp |
| OppA rabbit | Rabbit | 1:100 | Obtained from our own lab |
| Alexa Fluor 488 | Goat anti-rabbit | 1:200 | Invitrogen |
| Alexa Fluor 488 | Donkey anti-mouse | 1:500 | Invitrogen |
| Cy3 | Donkey anti-mouse | 1:50 | Jackson immunoresearch laboratories |
| Cy3 | Monkey anti-goat | 1:100 | Santa cruz biotechnology |