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. 2020 Jan 15;10:348. doi: 10.1038/s41598-019-57185-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Trabecular bone in the distal femur with fluorescent labels (calcein and alizarin) in (A) male wild type (WT), (B) male Keap1 heterozygous (Ht), (C) female wild type (WT) and (D) female Keap1 heterozygous (Ht) mice, indicating mineralizing surface (MS/BS), mineral appositional rate (MAR) and bone formation rate (BFR/BS). There was no difference in MS/BS, MAR and BFR between WT and Ht mice in trabecular bone. Data were tested using two-way ANOVA with Keap1 genotype and gender as main effects.