Table 1.
Fatty acid composition and total menaquinone content of S. xylosus strains related to growth temperature.
Strain | DSM 20266 T | J70 | J70 + 5% NaCl | |||
---|---|---|---|---|---|---|
Growth temperature Fatty acid (%) | 10 °C | 30 °C | 10 °C | 30 °C | 10 °C | 30 °C |
14:0 iso | 0.6 ± 0.0 | 5.0 ± 0.3 | 0.5 ± 0.1 | 5.9 ± 0.2 | 2.0 ± 0.3 | 4.0 ± 0.3 |
14:0 | n.d. | 0.7 ± 0.1 | n.d. | n.d. | n.d. | n.d. |
15:0 iso | 9.4 ± 0.1 | 18.2 ± 1.4 | 13.9 ± 0.3 | 19.0 ± 0.3 | 17.4 ± 1.6 | 18.7 ± 1.1 |
15:0 anteiso | 71.3 ± 1.2 | 56.2 ± 1.7 | 68.0 ± 2.4 | 53.3 ± 0.7 | 70.5 ± 2.8 | 58.1 ± 4.2 |
16:0 iso | 1.1 ± 0.1 | 4.4 ± 0.3 | 2.4 ± 0.1 | 5.5 ± 0.3 | 2.6 ± 0.5 | 3.8 ± 0.2 |
16:0 | n.d. | 1.9 ± 0.2 | n.d. | 1.8 ± 0.0 | n.d. | 1.7 ± 0.2 |
17:0 iso | 1.5 ± 0.1 | 3.6 ± 0.1 | 2.6 ± 0.1 | 4.3 ± 0.1 | 1.9 ± 0.5 | 4.0 ± 0.7 |
17:0 anteiso | 12.4 ± 0.2 | 3.8 ± 0.6 | 10.1 ± 0.4 | 3.6 ± 0.0 | 4.5 ± 0.6 | 3.9 ± 0.5 |
18:0 | 1.2 ± 0.9 | 4.2 ± 2.2 | 2.6 ± 0.2 | 3.7 ± 0.1 | 0.7 ± 0.1 | 4.1 ± 1.4 |
20:0 | 2.5 ± 0.3 | 1.4 ± 0.2 | n.d. | 3.8 ± 1.0 | n.d. | 1.7 ± 0.4 |
WAMT (°C) | 31.2 | 37.4 | 32.3 | 38.8 | 31.9 | 37.1 |
ΔWAMT (°C) | 6.2 | 6.5 | 5.2 | |||
Menaquinone concentration (nmol/g cell wet weight) | 217 ± 10 | 250 ± 5 | 254 ± 14 | 296 ± 8 | n.a. | n.a. |
Temperature adaptations are presented as weighted average melting temperature (WAMT) of the profiles (n.d.: not detected; n.a.: not analysed). Numbers following ± sign are standard deviations.